The NFL also said in a statement Wednesday that after investigating the Chargers' use of towels that included an adhesive substance, it was determined that San Diego did not violate any rules.
After consulting with the Competition Committee, the league said it has advised all clubs that the use of towels or other products that contain any type of adhesive substance is prohibited on game days until further notice.
The Chargers said they will appeal the fine.
"It's disappointing to me," coach Norv Turner said. "I think the most important thing, obviously, is that there's a lot of people in this league that were using the towel and certainly it wasn't being done in any way to deceive anybody."
In a statement, the Chargers said they were appealing the fine because their staff member "was unaware that the game official was trying to get his attention and he cooperated fully once he became aware."