The seven-time Grand Slam title winner was scheduled to play for the Washington Kastles in home matches Monday and Tuesday, and on the road against the Texas Wild on Wednesday. The Kastles are on a 32-match winning streak.
Williams has not competed since a first-round loss at the French Open on May 26. The 33-year-old American had played at Wimbledon every year since making her debut there in 1997.
The Kastles announced Friday that Martina Hingis would replace Williams in their lineup.